Leachables – what are they doing to your data?
Leachables are compounds that leach from plastic containers or coatings of a container, generally in response to the direct contact with the sample, reagent or solution stored in the container. Leachables can arise from the container itself or the closure (cap or liner/seal). While the effect of leachables on pharmaceuticals has been understood and regulated [...]
Isn’t one polypropylene vial the same as another?
Is one plastic tube the same as any other? NO! Even when you subtract the differences between closure type and bottom finishes, there are inherent differences in the plastic resin that is used to manufacture vials. Most notably, even when the same resin type is used (polypropylene vs. polycarbonate vs. polystyrene, for example), different resin [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONImmunohistochemistry – how to get great pictures from your tissue
Do you want awesome immunohistochemical images, with 2, 3 or even 4 colors to demonstrate your proteins/structures/cells of interest? Do your antibodies and formalin or paraformaldehyde not play well together? Don’t fret, with the right tissue processing and storage conditions, you can get beautiful tissue sections with multiple stains from frozen sections. The first step [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, ihc, immunihistochemistry, leak, leakage, leaking, LN2, Pressure, WHEATONSterile is not pyrogen-free… understanding the difference
Sterile vs. pyrogen-free – it’s important to your work and you need to know the difference. First of all, what is a pyrogen? Simply, a pyrogen is any substance that causes a fever. The most commonly thought of pyrogens are bacterial endotoxins and exotoxins, although the host body (human or animal) can also produce pyrogens. [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONIntegration of barcoding decreases time, cost and bio-sample loss
Because of this expanded need, many institutions, from government institutions, medical centers, and research academic institutions as well as pharmaceutical and biotech companies are creating large bio-sample libraries. Bio-samples are also being shipped from multiple locations around the world to consolidated storage facilities, sometimes within a single country but more and more often on a [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONPop goes that (cryo)tube…
To avoid cryotube or cryovial explosion, remember to use quality vials and secure the cap. With ultra-low temperature (ULT) sample storage, one of the most preventable ways that precious samples get damaged or lost occurs when excess pressure builds up inside of a cryogenic vial or glass ampule during freeze/thaw cycles. Although the cap seal [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONHow two simple tips can prevent your vials from exploding!
Two simple rules to prevent leakage of your vital samples: Secure the cap seal and do not overfill the vial. With ultra-low temperature (ULT) sample storage, one of the most preventable ways that precious samples get damaged or lost occurs when excess pressure builds up inside of a cryogenic vial or glass ampule during freeze/thaw [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONA review of cryopreservation: from the point of view of others.
This week, we here at CryoFEED decided to share a few papers with our readers that detail some of the various issues with cryopreservation and why understanding these issues is important for all of those involved in the biobanking process. First, is a review by John G Baust, Dayong Gao, and John M Baust: Cryopreservation: [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONWhy use a barcode for the samples in our biobank?
One of the most important regulatory requirements for biobanks is maintaining the confidentiality of the individuals whose samples reside in the biobank. The amount of information collected to correlate with the specific sample is dependent on the informed consent that is obtained. Samples can be collected and labeled in the following methods: Anonymous – Samples [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATONHow to integrate barcoding into my biobank or library?
You know that adding barcoding to your sample library will ease sample tracking and recovery, as well as Q&A, regulations, and more down the road. But how are you going to implement this into your current system? First off, don’t wait. The more samples that are added to your biobank or sample library that are [...]
Tags: CryoELITE, cryogenic, cryopreservation, cryopreservative, Cryostorage, cryotube, cryovial, explosion, leak, leakage, leaking, LN2, Pressure, WHEATON
